deep freezer

The cooling rate of skin in an-80 ℃ deep freezer was 1-3 ℃/ min, and it was more gradual and steady than in a dry-ice container.

-80℃低温冰箱能使皮片以每分钟1～3℃的速度降温，比应用干冰降温的较平稳。

Methods: The liver and spleen fractions of mice infected by trypanosoma evansi were stored at-20 ℃ and-80 ℃ in ordinary refrigerator or deep freezer for 7,10 and 60 days, respectively.

方法：感染伊氏锥虫小鼠的肝和脾组织分别冻存于-20℃和-80℃低温冰箱7、10、60d，观察复苏后伊氏锥虫的形态、活力及对小鼠的感染力。

Cryo immunotherapy group: On day 0, the mice were treated once by deep freezer.

1冷冻免疫组：第0天，用深部冷冻治疗仪治疗荷瘤鼠1次。